Animal Cell Labeled Biography
Source:- Google.com.pk
FSL Constructs for Labeling of Cells and Real-Time Imaging
Rapid Nontoxic in vitro/in vivo Labeling Without Loss of Function or Vitality
FSL Constructs are compounds based on KODE™ technology designed to label hydrophobic surfaces including living cells with carbohydrates, biotin, fluorophores, or 125I.1 All KODE FSL Constructs consist of three essential designable features:
a functional component (F)
a spacer (S)
and a diacyl lipid (L)
FSL Constructs disperse in water for biocompatibility and spontaneously and stably incorporate into cell membranes.
Cells modified with KODE constructs are known as kodecytes2 and kodevirions3 and are still vital and functional after labeling. Kodecytes and kodevirions retain their normal functionality while gaining the functionality introduced by the FSL Construct. FSL Constructs can also be used to modify other hydrophobic surfaces including fixed cells and solid-phase surfaces including silica, nitrocellulose, and paper.4
FSL Constructs have been used in vitro to modify embryos, spermatozoa, zebrafish5, epithelial/endometrial cells, red blood cells,2,6 and virions.3,7 Kodecytes have been infused intravascularly into experimental animals in vivo without loss of vitality.8,9
An improved method to screen Fc function of immunoglobulin products was developed using CMV kodecytes,10 and FSL antigen constructs have been printed onto silica to create a convenient array for antibody identification.4 Measles virions (MV) were fluorescently labeled to monitor binding to DC-SIGN+ expressing CHO cells using flow cytometry.7 Carbohydrate FSL Constructs11 have been used to modify cells and viruses to study virus inhibition,12 identify Lewis antibody,13 prepare labeled cells for ABO QC methods,2,13 and tag cells for flow cytometry.6
The combination of dispersibility in biocompatible media, spontaneous incorporation into cell membranes, and apparent low toxicity, makes FSL Constructs a valuable research tool for the development of new cell biology applications.
View the Journal of Visualized Experiments (JoVE) video on (KODE technology).
References
1. FSL Constructs: A simple method for modifying cell/virion surfaces with a range of biological markers without affecting their viability. Blake, D.A., Bovin, N.V., Bess, D., and Henry, S.M., J. Visualized Experiment (JoVE), 54, e3289 (2011) (Video article).
2. Henry, S.M., Modification of red blood cells for laboratory quality control use. Curr. Opin. Hematol. 16, 467-72 (2009).
3. Fluorescein and radiolabeled Function-Spacer-Lipid constructs allow for simple in vitro and in vivo bioimaging of enveloped virions. Hadac, E.M., Federspiel, M.J., Chernyy, E., Tuzikov, A., Korchagina, E., Bovin, N.V., Russell, S. and Henry, S.M., J. Viriological Methods, 176, 78-84 (2011).
4. Inkjet printing of Function-Spacer-Lipid (FSL) biomolecules, Barr, K., Diegel, O., Parker, S., and Henry, S., NZ Rapid Product Development Conference 2011, Auckland University of Technology, Auckland, New Zealand.
5. Fluorescent Function-Spacer-Lipid construct labeling allows for real-time in vivo imaging of cell migration and behavior in zebrafish. Lan, C.-C., Blake, D., Henry, S., and Love, D.F., J. Fluorescence, 22, 1055-63 (2012).
6. Flow cytometry evaluation of red blood cells mimicking naturally-occurring ABO subgroups following modification with variable amounts of FSL-A and B constructs. Hult, A.K., Frame, T., Chesla, S., Henry, S., and Olsson, M.L. Transfusion, 52, 247-51 (2011).
7. A prominent role for DC-SIGN+ dendritic cells in initiation and dissemination of measles virus infection in non-human primates. Mesman, A.W., de Vries, R.D., Duprex, W.P., de Swart, R.L., and Geijtenbeek, T.B.H., PLoS One, 7, e49573 (2012).
8. Modeling transfusion reactions and predicting in vivo cell survival with kodecytes. Oliver, C., Blake, D., and Henry, S., Transfusion, 51, 1723-30 (2011).
9. In vivo neutralization of anti-A and successful transfusion of A antigen-incompatible red blood cells in an animal model. Oliver, C., Blake, D., and Henry, S., Transfusion, 51, 2664-75 (2011).
10. An improved Fc function assay utilizing CMV antigen-coated red blood cells generated with synthetic function-spacer-lipid constructs. Georgakopoulos, T., Komarraju, S., Henry, S., and Bertolini, J., Vox Sanguinis, 102, 72-8 (2012).
11. Toward creating cell membrane glyco-landscapes with glycan lipid constructs. Korchagina, E., Tuzikov, A., Formanovsky, A., Popova, I., Henry, S., and Bovin, N., Carbohydrate Res., 356, 238-46 (2012).
12. A synthetic globotriaosylceramide analogue inhibits HIV-1 infection in vitro by two mechanisms. Harrison, A.L., Olsson, M.L., Brad Jones, R., Ramkumar, S., Sakac, D., Binnington, B., Henry, S., Lingwood, C.A., and Branch, D.R., Glycoconjugate J., 27, 515-524 (2010)
- See more at: http://www.sigmaaldrich.com/life-science/proteomics/proteomics-products.html?TablePage=106348994&%3Bcm_mmc=Google_Biochemistry-_-search-_-KODE_KODE-_-cell+labeling_Phrase&%3Bcm_guid=1-_-100000000000000036736-_-9147164840&cm_mmc=Google_Biochemistry-_-search-_-KODE_KODE-_-cell+labeling_Phrase&cm_guid=1-_-100000000000000036736-_-9147164840#sthash.CiROG6fl.dpuf
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
Animal Cell Labeled Animal Cell Model Diagram Project Parts Structure Labeled Coloring and Plant Cell Organelles Cake
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